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The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

DOI:

04:50 min

February 08, 2022

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Chapters

  • 00:00Introduction
  • 00:30Glycogen Extraction
  • 02:44Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen
  • 04:25Conclusion

Summary

自动翻译

An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.

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