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Isolamento rápido de células únicas de dentes humanos e de camundongos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Rapid Isolation of Single Cells from Mouse and Human Teeth

Isolamento rápido de células únicas de dentes humanos e de camundongos

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3,125 Views

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06:09 min

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October 28, 2021

DOI:

10.3791/63043-v

DOI:
10.3791/63043-v

•

06:09 min
October 28, 2021

•

5 Views
, , ,
1Department of Histology and Embryology, Faculty of Medicine,Masaryk University, 2Department of Molecular Neuroimmunology, Centre for Brain Research,Medical University of Vienna, 3Department of Physiology and Pharmacology,Karolinska Institute

Summary

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O protocolo atual apresenta um método rápido, eficiente e suave para isolar células únicas adequadas para análise de RNA-seq unicelular a partir de um incisivo de rato em crescimento contínuo, molar de rato e dentes humanos.

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Cite this Article

Krivanek, J., Lavicky, J., Bouderlique, T., Adameyko, I. Rapid Isolation of Single Cells from Mouse and Human Teeth. J. Vis. Exp. (176), e63043, doi:10.3791/63043 (2021).

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