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Rapid Isolation of Single Cells from Mouse and Human Teeth
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

3,102 Views

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06:09 min

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October 28, 2021

DOI:

10.3791/63043-v

DOI:
10.3791/63043-v

•

06:09 min
October 28, 2021

•

5 Views
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1Department of Histology and Embryology, Faculty of Medicine,Masaryk University, 2Department of Molecular Neuroimmunology, Centre for Brain Research,Medical University of Vienna, 3Department of Physiology and Pharmacology,Karolinska Institute

Summary

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The current protocol presents a fast, efficient, and gentle method for isolating single cells suitable for single-cell RNA-seq analysis from a continuously growing mouse incisor, mouse molar, and human teeth.

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Cite this Article

Krivanek, J., Lavicky, J., Bouderlique, T., Adameyko, I. Rapid Isolation of Single Cells from Mouse and Human Teeth. J. Vis. Exp. (176), e63043, doi:10.3791/63043 (2021).

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