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Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

JoVE Journal
Neuroscience
Author Produced

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JoVE Journal Neuroscience

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

4,151 Views

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09:50 min

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December 25, 2021

DOI:

10.3791/63038-v

DOI:

10.3791/63038-v

•

09:50 min

December 25, 2021

•

16 Views

Iskalen Cansu Topcu Okan², Mehri Ahmadian², Yesim Tutuncu², Halit Yusuf Altay², Cavit Agca²

¹Molecular Biology, Genetics and Bioengineering Program,Sabanci University, ²Nanotechnology Research and Application Center (SUNUM),Sabanci University

Tags

Digital Droplet PCR AAV Transduction Murine Retina Quantification Absolute Quantification CRISPR Gene Therapy Mitochondrial DNA Transfection Virus Purification

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