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Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina
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Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

4,216 Views

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09:50 min

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December 25, 2021

DOI:

10.3791/63038-v

DOI:
10.3791/63038-v

•

09:50 min
December 25, 2021

•

14 Views
, , , ,
1Molecular Biology, Genetics and Bioengineering Program,Sabanci University, 2Nanotechnology Research and Application Center (SUNUM),Sabanci University

Summary

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This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.

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Digital Droplet PCRAAV TransductionMurine RetinaQuantificationAbsolute QuantificationCRISPRGene TherapyMitochondrial DNATransfectionVirus Purification

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Okan, I. C. T., Ahmadian, M., Tutuncu, Y., Altay, H. Y., Agca, C. Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina. J. Vis. Exp. (178), e63038, doi:10.3791/63038 (2021).

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