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Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels
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Medicine
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JoVE Journal Medicine
Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

DOI:
10.3791/63009-v

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04:35 min

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October 07, 2021

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, , , , ,
1Department of Molecular Physiology,National Cerebral and Cardiovascular Center Research Institute, 2International Research Center for Medical Sciences (IRCMS),Kumamoto University, 3Department of Cardiovascular Medicine, Graduate School of Medical Sciences,Kumamoto University, 4X-Earth Center, Faculty of Advanced Science and Technology,Kumamoto University

Chapters

  • 00:04Introduction
  • 00:46Perfusion
  • 02:56Results: 3D Evaluation of Murine Blood Vessels in The Lower Extremities of the Mouse
  • 03:58Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

Tags

Computed Tomography3D VisualizationMurine Hind-limb VesselsSample PreparationHeparinVasodilation BufferParaformaldehydeContrast MediumPerfusionHind-limb Ischemia ModelFemoral ArteryFemoral VeinCollateral Vessels

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