/

/

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

2,047 Views

•

04:35 min

•

October 07, 2021

DOI:

10.3791/63009-v

DOI:

10.3791/63009-v

•

04:35 min

October 07, 2021

•

1 Views

Daiki Seya¹, Yuqing Xu^2,3, Toshifumi Mukunoki⁴, Kenichi Tsujita³, Osamu Nakagawa¹, Yuichiro Arima^1,2,3,4

¹Department of Molecular Physiology,National Cerebral and Cardiovascular Center Research Institute, ²International Research Center for Medical Sciences (IRCMS),Kumamoto University, ³Department of Cardiovascular Medicine, Graduate School of Medical Sciences,Kumamoto University, ⁴X-Earth Center, Faculty of Advanced Science and Technology,Kumamoto University

Summary

Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

Explore More Videos

Computed Tomography 3D Visualization Murine Hind-limb Vessels Sample Preparation Heparin Vasodilation Buffer Paraformaldehyde Contrast Medium Perfusion Hind-limb Ischemia Model Femoral Artery Femoral Vein Collateral Vessels

Read Article