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从新生小鼠中磁分离小胶质细胞用于原代细胞培养
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
DOI:

07:23 min

July 25, 2022

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Chapters

  • 00:05Introduction
  • 00:32Brain Dissection
  • 01:29Brain Dissociation and Magnetic Microglial Isolation
  • 04:23Phagocytic Assay
  • 05:03Results: Phagocytic Activity and Magnetic Isolation of Microglia In Vitro
  • 06:49Conclusion

Summary

Automatic Translation

原代小胶质细胞培养通常用于评估新的抗炎分子。本协议描述了一种可重复且相关的方法,用于从新生儿幼崽中磁性分离小胶质细胞。

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