Here, we present a protocol to isolate and purify primary microglia in animal models of demyelinating diseases, utilizing columnar magnetic-activated cell sorting.
Zhang, H., Yang, S., Chen, M., Tian, D., Qin, C. Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination. J. Vis. Exp. (182), e63511, doi:10.3791/63511 (2022).