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Journal
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Biochemistry
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双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal
Biochemistry
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Please note that all translations are automatically generated.
Click here for the English version.
双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学
DOI:
10.3791/62954-v
•
14:12 min
•
December 11, 2021
•
Katherina Hemmen*
1
,
Susobhan Choudhury*
1
,
Mike Friedrich
,
Johannes Balkenhol
,
Felix Knote
,
Martin J. Lohse
,
Katrin G. Heinze
1
Rudolf Virchow Center for Integrative and Translation Bioimaging
,
Julius-Maximilian University Wuerzburg
,
2
Max Delbrück Center for Molecular Medicine, Berlin
Chapters
00:05
Introduction
01:09
Sample Preparation
04:34
Calibration Measurements
05:58
Live Cell Measurements
06:52
Data Export
07:23
Calibration Measurements
09:20
Live Cell Experiments
11:20
Results
13:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种实验方案和数据分析工作流程,以执行活细胞双色荧光交叉相关光谱(FCCS)与Förster共振能量转移(FRET)相结合,以使用现代荧光标记技术研究活细胞中的膜受体动力学。
Tags
Fluorescence Correlation Spectroscopy
Cross-correlation Spectroscopy
FRET
G Protein-coupled Receptors
Beta-2 Adrenergic Receptors
Sphingolipid Receptors
Protein-protein Interaction
Protein Dynamics
Live Cells
Cell Culture
Transfection
SNAP-tag
Fluorescence Anisotropy
Translational Diffusion
Rotational Diffusion
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