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生物化学
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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
JoVE 杂志
生物化学
Author Produced
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JoVE 杂志 生物化学
Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

4,555 Views

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14:12 min

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December 11, 2021

DOI:

10.3791/62954-v

DOI:
10.3791/62954-v

•

14:12 min
December 11, 2021

•

20 Views
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1Rudolf Virchow Center for Integrative and Translation Bioimaging,Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin

Summary

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We present an experimental protocol and data analysis workflow to perform live cell dual-color fluorescence cross correlation spectroscopy (FCCS) combined with Förster Resonance Energy transfer (FRET) to study membrane receptor dynamics in live cells using modern fluorescence labeling techniques.

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Hemmen, K., Choudhury, S., Friedrich, M., Balkenhol, J., Knote, F., Lohse, M. J., Heinze, K. G. Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells. J. Vis. Exp. (178), e62954, doi:10.3791/62954 (2021).

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