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使用多重原位杂交检测小鼠迷走神经传入神经元中G蛋白偶联受体表达

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Please note that all translations are automatically generated. Click here for the English version.

使用多重原位杂交检测小鼠迷走神经传入神经元中G蛋白偶联受体表达

DOI:

10.3791/62945-v

•

08:16 min

•

September 20, 2021

•

Johnson Bob-Manuel, Laurent Gautron

¹Center for Hypothalamic Research and Department of Internal Medicine,UTSouthwestern Medical Center at Dallas

Chapters

00:04Introduction
00:39The Mouse Jugular-nodose Ganglia Sample Collection
02:51Pretreatment of the Slides and In Situ Hybridization (ISH)
04:51Microscopy and Data Analysis
05:34Results: Microscopic Analysis of the Mouse Vagal Afferent Neurons After Multiplex in Situ Hybridization
07:34Conclusion

Summary

Automatic Translation

采用多重原位杂交（ISH）来同时可视化成年小鼠整个迷走神经节复合物中两个G蛋白偶联受体和一个转录因子的转录物。该协议可用于生成迷走神经传入神经元转录谱的准确图谱。

Tags

Keywords: G Protein-coupled Receptor Vagal Afferent Neurons Multiplex In Situ Hybridization Mouse Transcriptional Profiling Cryosectioning Histological Techniques

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