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JoVE Journal Neuroscience
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
 

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Article DOI: 10.3791/62945-v • 08:16 min • September 20th, 2021
September 20th, 2021 •
1, 1
1Center for Hypothalamic Research and Department of Internal Medicine, UTSouthwestern Medical Center at Dallas

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Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent neurons.

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G Protein-coupled Receptor Expression Mouse Vagal Afferent Neurons Multiplex In Situ Hybridization Transcriptional Profile Heterogeneous Cell Types Visualization Accurate Maps Procedure Me Johnson Bob-manuel Research Technician Vagal Ganglionic Mass Dissecting Scope Surgical Instruments Decapitation Sternohyoid Muscle Omohyoid Muscle Occipital Bone Carotid Artery Vagus Nerve Hypoglossal Nerve Foramen Magnum Hypoglossal Nerve Cutting Nodose Ganglion Occipital Bone Breaking Jugular Ganglion Extraction
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