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光ピンセットを用いた閉じ込めにおける細胞下力学の直接力測定
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
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光ピンセットを用いた閉じ込めにおける細胞下力学の直接力測定

日本語

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4,285 Views

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09:56 min

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August 31, 2021

DOI:

10.3791/62865-v

DOI:
10.3791/62865-v

•

09:56 min
August 31, 2021

•

25 Views
, , , ,
1Neurophotonics and Mechanical Systems Biology,Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)

Summary

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ここでは、光学トラップによる直接力測定を用いて、3次元閉じ込めで単離した胚ゼブラフィッシュ細胞の細胞内の機械的特性を調べるプロトコルを提示する。

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Cite this Article

Català-Castro, F., Venturini, V., Ortiz-Vásquez, S., Ruprecht, V., Krieg, M. Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers. J. Vis. Exp. (174), e62865, doi:10.3791/62865 (2021).

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