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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

4,284 Views

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09:56 min

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August 31, 2021

DOI:

10.3791/62865-v

DOI:
10.3791/62865-v

•

09:56 min
August 31, 2021

•

25 Views
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1Neurophotonics and Mechanical Systems Biology,Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)

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Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.

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Català-Castro, F., Venturini, V., Ortiz-Vásquez, S., Ruprecht, V., Krieg, M. Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers. J. Vis. Exp. (174), e62865, doi:10.3791/62865 (2021).

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