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Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

English

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3,908 Views

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07:19 min

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August 04, 2021

DOI:

10.3791/62850-v

DOI:
10.3791/62850-v

•

07:19 min
August 04, 2021

•

33 Views
, , ,
1College of Medicine,Central Michigan University, 2Biochemistry, Cell and Molecular Biology Program,Central Michigan University, 3Neuroscience Program,Central Michigan University

Summary

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Bioluminescence-light emitted by a luciferase enzyme oxidizing a small-molecule substrate, a luciferin-can be harnessed to activate photosensory proteins, thereby adding another dimension to light stimulation and enabling the manipulation of a multitude of light-mediated functions in cells across temporal and spatial scales.

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Cite this Article

Crespo, E. L., Bjorefeldt, A., Prakash, M., Hochgeschwender, U. Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo. J. Vis. Exp. (174), e62850, doi:10.3791/62850 (2021).

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