ジャーナル
/
生化学
/
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
JoVE Journal
生化学
This content is Free Access.
JoVE Journal 生化学
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

DOI:
10.3791/62850-v

•

07:19 min

•

August 04, 2021

•

, , ,
1College of Medicine,Central Michigan University, 2Biochemistry, Cell and Molecular Biology Program,Central Michigan University, 3Neuroscience Program,Central Michigan University

章

  • 00:04Introduction
  • 00:40Bioluminescence Activation In Vitro
  • 03:26Bioluminescence Activation In Vivo
  • 04:49Results: Bioluminescence for Optogenomic Signaling, Transcription, and Manipulation
  • 06:34Conclusion

概要

自動翻訳

自動翻訳

Bioluminescence-light emitted by a luciferase enzyme oxidizing a small-molecule substrate, a luciferin-can be harnessed to activate photosensory proteins, thereby adding another dimension to light stimulation and enabling the manipulation of a multitude of light-mediated functions in cells across temporal and spatial scales.

タグ

BioluminescenceOptogeneticsPhotosensory ProteinsIn VitroIn VivoLuciferinCoelenterazine (CTZ)Cell CultureLive Cell ImagingRepeated Light StimulationPerfusion System

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Read Article