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Developmental Biology
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使用光遗传TAEL/C120系统在斑马鱼胚胎中的光诱导GFP表达
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Developmental Biology
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JoVE Journal
Developmental Biology
Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
Please note that all translations are automatically generated.
Click here for the English version.
使用光遗传TAEL/C120系统在斑马鱼胚胎中的光诱导GFP表达
DOI:
10.3791/62818-v
•
05:28 min
•
August 19, 2021
•
Jesselynn LaBelle
,
Stephanie Woo
1
Department of Molecular Cell Biology
,
University of California
Chapters
00:05
Introduction
00:45
Global Light Induction
01:26
Quantitative Real-time PCR and Fluorescence Microscopy
03:59
Results: Light-activated Gene Expression Assessment in Zebrafish Embryo
04:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
光遗传学是一种功能强大的工具,具有广泛的应用。该协议演示了如何使用蓝光响应 TAEL/C120 系统在斑马鱼胚胎中实现光诱导基因表达。
Tags
Light-induced GFP Expression
Optogenetic
TAEL/C120 System
Zebrafish Embryos
Inducible Gene Expression
Lineage Tracing
Gain-of-function Assays
Rescue Experiments
RNA Extraction
CDNA Synthesis
Quantitative PCR
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