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Rapid Characterization of Genetic Parts with Cell-Free Systems
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Rapid Characterization of Genetic Parts with Cell-Free Systems

Rapid Characterization of Genetic Parts with Cell-Free Systems

English

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1,469 Views

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05:00 min

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August 30, 2021

DOI:

10.3791/62816-v

DOI:
10.3791/62816-v

•

05:00 min
August 30, 2021

•

3 Views
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1US Army Combat Capabilities Development Command Army Research Laboratory, 2Biology and Biological Engineering,California Institute of Technology, 3Excet, Inc., 4US Army Combat Capabilities Development Command Chemical Biological Center

Summary

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Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.

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Cite this Article

McManus, J. B., Bernhards, C. B., Sharpes, C. E., Garcia, D. C., Cole, S. D., Murray, R. M., Emanuel, P. A., Lux, M. W. Rapid Characterization of Genetic Parts with Cell-Free Systems. J. Vis. Exp. (174), e62816, doi:10.3791/62816 (2021).

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