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Rapid Characterization of Genetic Parts with Cell-Free Systems
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Rapid Characterization of Genetic Parts with Cell-Free Systems

Rapid Characterization of Genetic Parts with Cell-Free Systems

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05:00 min

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August 30, 2021

DOI:

10.3791/62816-v

DOI:
10.3791/62816-v

•

05:00 min
August 30, 2021

•

3 Views
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1US Army Combat Capabilities Development Command Army Research Laboratory, 2Biology and Biological Engineering,California Institute of Technology, 3Excet, Inc., 4US Army Combat Capabilities Development Command Chemical Biological Center

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Well-characterized genetic parts are necessary for the design of novel genetic circuits. Here we describe a cost-effective, high-throughput method for rapidly characterizing genetic parts. Our method reduces cost and time by combining cell-free lysates, linear DNA to avoid cloning, and acoustic liquid handling to increase throughput and reduce reaction volumes.

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McManus, J. B., Bernhards, C. B., Sharpes, C. E., Garcia, D. C., Cole, S. D., Murray, R. M., Emanuel, P. A., Lux, M. W. Rapid Characterization of Genetic Parts with Cell-Free Systems. J. Vis. Exp. (174), e62816, doi:10.3791/62816 (2021).

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