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Humanized Mediator Release Assay as a Read-Out for Allergen Potency
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

English

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3,177 Views

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10:22 min

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June 29, 2021

DOI:

10.3791/62702-v

DOI:
10.3791/62702-v

•

10:22 min
June 29, 2021

•

15 Views
, , ,
1Department of Biosciences,University of Salzburg

Summary

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Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.

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Wenger, M., Bethanis, A., Johnson, L., Aglas, L. Humanized Mediator Release Assay as a Read-Out for Allergen Potency. J. Vis. Exp. (172), e62702, doi:10.3791/62702 (2021).

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