/
/
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
This content is Free Access.
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
3,120 Views
•
10:22 min
•
June 29, 2021
DOI:
Summary
Automatically generated
Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.
Explore More Videos
Read Article
Cite this Article
Wenger, M., Bethanis, A., Johnson, L., Aglas, L. Humanized Mediator Release Assay as a Read-Out for Allergen Potency. J. Vis. Exp. (172), e62702, doi:10.3791/62702 (2021).
Copy