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Cancer Research

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使用患者源性肿瘤类器官的高通量体外检测

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

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使用患者源性肿瘤类器官的高通量体外检测

DOI:

10.3791/62668-v

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06:25 min

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June 14, 2021

•

Arisa Higa, Nobuhiko Takahashi³, Gen Hiyama, Hirosumi Tamura, Hirotaka Hoshi, Kenju Shimomura, Shinya Watanabe, Motoki Takagi

¹FUJIFILM Wako Bio Solutions Corporation, ²Medical-Industrial Translational Research Center,Fukushima Medical University, ³Department of Bioregulation and Pharmacological Medicine,Fukushima Medical University

Chapters

00:04Introduction
00:59Patient-Derived Tumor Organoid Culture
02:25High Throughput Assay System for Growth Inhibition
03:32High Throughput Assay System with Cell Picking and Imaging for Growth Inhibition
04:30Results: Estimation of Anticancer Effects on the Patient-Derived Tumor Organoids
05:55Conclusion

Summary

Automatic Translation

开发了一种高精度 的体外 高通量测定系统，以使用患者来源的肿瘤类器官（PDO）评估抗癌药物，类似于癌症组织，但不适用于具有96孔和384孔板 的体外 高通量测定系统。

Tags

Patient-derived Tumor Organoids High-throughput In Vitro Assay Cell Analysis 2D Culture 3D Culture Molecular Targeted Drugs Cell Suspension 384-well Microplate Liquid Handler Cell Fragmentation And Dispersion Instrument

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