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Ensaio in vitro de alta produtividade usando organoides tumorais derivados do paciente
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

Ensaio in vitro de alta produtividade usando organoides tumorais derivados do paciente

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4,748 Views

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06:25 min

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June 14, 2021

DOI:

10.3791/62668-v

DOI:
10.3791/62668-v

•

06:25 min
June 14, 2021

•

4 Views
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1FUJIFILM Wako Bio Solutions Corporation, 2Medical-Industrial Translational Research Center,Fukushima Medical University, 3Department of Bioregulation and Pharmacological Medicine,Fukushima Medical University

Summary

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Um sistema de ensaio in vitro de alta throughput foi desenvolvido para avaliar drogas anticancerígenas usando organoides tumorais derivados do paciente (PDOs), semelhantes aos tecidos cancerígenos, mas são inadequados para sistemas de ensaio in vitro de alto rendimento com placas de 96 poços e 384 poços.

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Higa, A., Takahashi, N., Hiyama, G., Tamura, H., Hoshi, H., Shimomura, K., Watanabe, S., Takagi, M. High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids. J. Vis. Exp. (172), e62668, doi:10.3791/62668 (2021).

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