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Eine effiziente Methode zur gerichteten Hepatozyten-ähnlichen Zellinduktion aus menschlichen embryonalen Stammzellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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Eine effiziente Methode zur gerichteten Hepatozyten-ähnlichen Zellinduktion aus menschlichen embryonalen Stammzellen

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2,591 Views

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08:05 min

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May 06, 2021

DOI:

10.3791/62654-v

DOI:
10.3791/62654-v

•

08:05 min
May 06, 2021

•

3 Views
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1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Summary

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Dieses Manuskript beschreibt ein detailliertes Protokoll zur Differenzierung menschlicher embryonaler Stammzellen (hESCs) in funktionelle hepatozytenähnliche Zellen (HLCs) durch kontinuierliche Ergänzung von Activin A und CHIR99021 während der hESC-Differenzierung zu definitivem Endoderm (DE).

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Cite this Article

Zhou, Q., Xie, X., Zhong, Z., Sun, P., Zhou, X. An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells. J. Vis. Exp. (171), e62654, doi:10.3791/62654 (2021).

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