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Ultracentrifugación de gradiente de densidad para investigar el reciclaje endocítico en células de mamíferos

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

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Ultracentrifugación de gradiente de densidad para investigar el reciclaje endocítico en células de mamíferos

3,538 Views

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05:13 min

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June 30, 2021

DOI:

10.3791/62621-v

DOI:

10.3791/62621-v

•

05:13 min

June 30, 2021

•

13 Views

Wai Wa Ray Chan*¹, Yu Qi Zhai*¹, Kwok-Fai Lau

¹School of Life Sciences,The Chinese University of Hong Kong

Tags

Density Gradient Ultracentrifugation Endocytic Recycling Mammalian Cells Protein Trafficking Recycling Endosomes Cell Fractionation Sucrose Gradient Post-nuclear Supernatant Homogenization Buffer Dounce Homogenizer Protease Inhibitor Phosphatase Inhibitor

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