ジャーナル
/
生化学
/
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells

DOI:
10.3791/62621-v

•

05:13 min

•

June 30, 2021

•

, ,
1School of Life Sciences,The Chinese University of Hong Kong

章

  • 00:05Introduction
  • 00:33Mammalian Cell Culture, Transfection and Cell Harvest
  • 02:07Density Gradient Column Preparation
  • 02:46Fractionation and Recovery of Recycling Endosome-Containing Fraction
  • 03:17Results: Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
  • 04:47Conclusion

概要

自動翻訳

自動翻訳

This paper aims to present a protocol for preparing recycling endosomes from mammalian cells using sucrose density gradient ultracentrifugation.

タグ

Density Gradient UltracentrifugationEndocytic RecyclingMammalian CellsProtein TraffickingRecycling EndosomesCell FractionationSucrose GradientPost-nuclear SupernatantHomogenization BufferDounce HomogenizerProtease InhibitorPhosphatase Inhibitor

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Protein Complex Affinity Capture from Cryomilled Mammalian Cells

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

Polysome Profiling without Gradient Makers or Fractionation Systems

Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Read Article