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全山神経筋接合部の免疫蛍光解析と形態測定解析のための単一骨格筋線維の解剖
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Neuroscience
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JoVE Journal Neuroscience
Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions
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全山神経筋接合部の免疫蛍光解析と形態測定解析のための単一骨格筋線維の解剖

日本語

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7,681 Views

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08:41 min

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August 14, 2021

DOI:

10.3791/62620-v

DOI:
10.3791/62620-v

•

08:41 min
August 14, 2021

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11 Views
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1Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine,Universidad de la República, 2Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE),Ministerio de Educación y Cultura

Summary

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神経筋接合成分を正確に検出する能力は、病理学的または発達的プロセスのために、そのアーキテクチャの変更を評価する上で非常に重要です。ここでは、定量的測定を行うために使用できる全マウント神経筋接合部の高品質画像を得るための簡単な方法の完全な説明を提示する。

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Cite this Article

Bolatto, C., Olivera-Bravo, S., Cerri, S. Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions. J. Vis. Exp. (174), e62620, doi:10.3791/62620 (2021).

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