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Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

DOI:
10.3791/62620-v

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08:41 min

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August 14, 2021

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, ,
1Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine,Universidad de la República, 2Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE),Ministerio de Educación y Cultura

Chapters

  • 00:17Introduction
  • 00:45Muscle Dissection
  • 04:43Teased Fiber Preparation
  • 06:44Results: Morphometric Evaluation of Whole-Mount Neuromuscular Junctions
  • 08:06Conclusion

Summary

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The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.

Tags

Keywords: Skeletal Muscle FibersImmunofluorescentMorphometric AnalysesWhole-mount Neuromuscular JunctionsEDL MuscleSoleus MuscleDissectionSurgical InstrumentsFixation

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