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September 07, 2021
DOI:
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology,Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education,Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research,Yikon Genomics Co. Ltd.
Summary
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本研究は、使用済み培地を使用したヒト胚の染色体スクリーニングのプロトコルを報告し、胚生検を回避し、NGSを使用して染色体倍数性を同定できるようにします。本稿では、培地の調製、全ゲノム増幅(WGA)、次世代シーケンシング(NGS)ライブラリの調製、データ解析など、詳細な手順を紹介します。
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Huang, J., Yao, Y., Jia, J., Zhu, X., Ma, J., Wang, J., Liu, P., Lu, S. Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis. J. Vis. Exp. (175), e62619, doi:10.3791/62619 (2021).
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