このコンテンツを視聴するには、JoVE 購読が必要です。 サインイン又は無料トライアルを申し込む。
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
1,791 Views
•
12:32 min
•
September 07, 2021
DOI:
1Centre for Reproductive Medicine, Department of Obstetrics and Gynecology,Peking University Third Hospital, 2National Clinical Research Center for Obstetrics and Gynecology(Peking University Third Hospital), 3Ministry of Education,Key Laboratory of Assisted Reproduction (Peking University), 4Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, 5Department of Clinical Research,Yikon Genomics Co. Ltd.
概要
Automatically generated
The present study reports a protocol for chromosome screening of human embryos that uses spent culture medium, which avoids embryo biopsy and enables chromosome ploidy identification using NGS. The present article presents the detailed procedure, including the preparation of culture medium, whole genome amplification (WGA), next-generation sequencing (NGS) library preparation, and data analysis.
Read Article
Cite this Article
Huang, J., Yao, Y., Jia, J., Zhu, X., Ma, J., Wang, J., Liu, P., Lu, S. Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis. J. Vis. Exp. (175), e62619, doi:10.3791/62619 (2021).
Copy