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使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量
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Biochemistry
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JoVE Journal Biochemistry
Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
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使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量

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3,041 Views

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11:38 min

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September 14, 2021

DOI:

10.3791/62608-v

DOI:
10.3791/62608-v

•

11:38 min
September 14, 2021

•

6 Views
, ,
1Institute for Chemistry and Biochemistry,Freie Universität Berlin, 2International Max-Planck Research School for Biology and Computation

Summary

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在这里，我们建立了一个方案，使用暴露于病理和生理流体剪切应激条件下的内皮细胞中的接近连接测定（PLA）同时可视化和分析多个SMAD复合物。

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Cite this Article

Mendez, P., Obendorf, L., Knaus, P. Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay. J. Vis. Exp. (175), e62608, doi:10.3791/62608 (2021).

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