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Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
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Biochemie
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JoVE Journal Biochemie
Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

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11:38 min

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September 14, 2021

DOI:

10.3791/62608-v

DOI:
10.3791/62608-v

•

11:38 min
September 14, 2021

•

6 Views
, ,
1Institute for Chemistry and Biochemistry,Freie Universität Berlin, 2International Max-Planck Research School for Biology and Computation

Summary

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Here, we establish a protocol to simultaneously visualize and analyze multiple SMAD complexes using proximity ligation assay (PLA) in endothelial cells exposed to pathological and physiological fluid shear stress conditions.

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TGF-β/BMP/SMAD SignalingProximity Ligation AssayFluid Shear StressMicrofluidic Flow ChannelsHUVEC CellsTranscription Factor ComplexesVascular Biology

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Mendez, P., Obendorf, L., Knaus, P. Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay. J. Vis. Exp. (175), e62608, doi:10.3791/62608 (2021).

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