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Probenvorbereitung zur bioinformatischen Analyse der DNA-Methylierung: Assoziationsstrategie für Adipositas und verwandte Merkmalsstudien
JoVE Journal
Genetics
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JoVE Journal Genetics
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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Probenvorbereitung zur bioinformatischen Analyse der DNA-Methylierung: Assoziationsstrategie für Adipositas und verwandte Merkmalsstudien

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3,928 Views

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14:56 min

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May 06, 2022

DOI:

10.3791/62598-v

DOI:
10.3791/62598-v

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14:56 min
May 06, 2022

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3 Views
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1Department of Internal Medicine, Ribeirão Preto Medical School,University of São Paulo, 2Centre for Computational Biology,University of Birmingham, 3Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences,University of Birmingham, 4Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine,The University of Edinburgh, 5Ribeirão Preto School of Physical Education and Sport,University of São Paulo, 6Health Sciences Department, Ribeirao Preto Medical School,University of Sao Paulo, 7Faculty of Pharmaceutical Sciences,University of São Paulo, 8Department of Genetics, Ribeirão Preto Medical School,University of São Paulo, 9Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto,University of São Paulo

Summary

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Die vorliegende Studie beschreibt den Workflow zur Verwaltung von DNA-Methylierungsdaten, die durch Microarray-Technologien erhalten werden. Das Protokoll veranschaulicht die Schritte von der Probenvorbereitung bis zur Datenanalyse. Alle Verfahren werden detailliert beschrieben, und das Video zeigt die wesentlichen Schritte.

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Noronha, N. Y., da Silva Rodrigues, G., de Souza Pinhel, M. A., Cazier, J., Watanabe, L. M., Menezes, A. N., Bueno, C. R., Nicoletti, C. F., de Oliveira, B. A. P., Schineider, I. M., Yonehara Noma, I. H., Dias Alcarás, I. C., Barbosa, F., Barbosa Nonino, C. Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies. J. Vis. Exp. (183), e62598, doi:10.3791/62598 (2022).

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