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Biology
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建立稳定的双生糖核糖核关系从口腔
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
Please note that all translations are automatically generated.
Click here for the English version.
建立稳定的双生糖核糖核关系从口腔
DOI:
10.3791/62484-v
•
07:32 min
•
April 13, 2021
•
Andrew J. Collins*
1,2
,
Pallavi P. Murugkar*
1,2
,
Floyd E. Dewhirst
2
1
The Forsyth Institute
,
2
Harvard School of Dental Medicine
Chapters
00:04
Introduction
01:11
Preparation Initiating Host Cultures and Sterilizing Equipment
01:55
Obtaining Sample of Oral Bacteria
02:30
Preparation of Filtrate from Oral Sample
03:28
Concentration of the Saccharibacteria Cells by Centrifugation
04:02
Infection of Host Cultures with Saccharibacteria-enriched Filtrate
04:47
Confirmation of Infection by PCR and Verification of Purity
05:36
Results: Confirmation of Infection and Purity of Binary Cultures of Symbiotic Saccharibacteria
06:54
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们演示了一种通过过滤牙菌斑和与宿主细菌共同培养来隔离新型细菌植物(糖化物)难以生长的成员的方法。
Tags
Saccharibacteria
Oral Cavity
Binary Co-culture
Obligate Parasites
Host Bacteria
Arachnia Propionica
Dental Plaque
Filtration
Isolation
Microbiological Techniques
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