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Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity
JoVE 杂志
生物学
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JoVE 杂志 生物学
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

DOI:
10.3791/62484-v

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07:32 min

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April 13, 2021

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, ,
1The Forsyth Institute, 2Harvard School of Dental Medicine

Chapters

  • 00:04Introduction
  • 01:11Preparation Initiating Host Cultures and Sterilizing Equipment
  • 01:55Obtaining Sample of Oral Bacteria
  • 02:30Preparation of Filtrate from Oral Sample
  • 03:28Concentration of the Saccharibacteria Cells by Centrifugation
  • 04:02Infection of Host Cultures with Saccharibacteria-enriched Filtrate
  • 04:47Confirmation of Infection by PCR and Verification of Purity
  • 05:36Results: Confirmation of Infection and Purity of Binary Cultures of Symbiotic Saccharibacteria
  • 06:54Conclusion

Summary

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We demonstrate a method for isolating difficult-to-grow members of the novel bacterial phylum, Saccharibacteria, by filtering dental plaque and co-culturing with host bacteria.

Tags

Keywords: SaccharibacteriaOral CavityBinary Co-cultureObligate ParasitesHost BacteriaArachnia PropionicaDental PlaqueFiltrationIsolationMicrobiological Techniques

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