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Journal
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Genetics
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マグナペアテオリザ
エにおけるクロマチン免疫沈降シーケンシング法を用いたヒストン修飾分布のゲノム解析
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Genetics
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JoVE Journal
Genetics
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in
Magnaporthe oryzae
Please note that all translations are automatically generated.
Click here for the English version.
マグナペアテオリザ
エにおけるクロマチン免疫沈降シーケンシング法を用いたヒストン修飾分布のゲノム解析
DOI:
10.3791/62423-v
•
09:25 min
•
June 02, 2021
•
Zechi Wu*
1
,
Wanyu Sun*
1
,
Sida Zhou
,
Li Zhang
,
Xinyu Zhao
,
Yang Xu
,
Weixiang Wang
1
Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy
,
Beijing University of Agriculture
Chapters
00:04
Introduction
00:39
Preparation of Protoplasts from
M. oryzae
01:48
In Vivo Crosslinking and Sonication
03:22
IP of Crosslinked Protein/DNA
04:23
Collecting and Rinsing the IP Products
05:04
Elution and Reverse Crosslinking of Protein/DNA Complexes
05:56
Purification and Recovery of DNA
06:51
DNA Repair and Solexa Library Construction
08:23
Results: Chromatin Immunoprecipitation Sequencing Method for Genome-wide Histone Modification Analysi
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、M.オリザエおよび他の糸状菌の病因における新しい標的遺伝子を同定することができるヒストン修飾のゲノム全体の分布を分析するプロトコルを提示する。
Tags
Chromatin Immunoprecipitation Sequencing (ChIP-seq)
Histone Modifications
Magnaporthe Oryzae
Epigenetic Regulation
Fungal Pathogenesis
Genome-wide Analysis
Transcription Factors
DNA-protein Interactions
Protoplast Preparation
Sonication
Agarose Gel Electrophoresis
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