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Quantitative Methoden zur Untersuchung der Protein-Arginin-Methyltransferase-1-9-Aktivität in Zellen
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Biology
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Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
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Quantitative Methoden zur Untersuchung der Protein-Arginin-Methyltransferase-1-9-Aktivität in Zellen

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3,763 Views

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08:11 min

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August 07, 2021

DOI:

10.3791/62418-v

DOI:
10.3791/62418-v

•

08:11 min
August 07, 2021

•

8 Views
, ,
1Structural Genomics Consortium,University of Toronto, 2Department of Pharmacology and Toxicology,University of Toronto

Summary

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Diese Protokolle liefern die Methodik zur Beurteilung der enzymatischen Aktivität einzelner Mitglieder der Protein-Arginin-Methyltransferase-Familie (PRMT) in Zellen. Detaillierte Richtlinien zur Bewertung der PRMT-Aktivität unter Verwendung endogener und exogener Biomarker, Methyl-Arginin-erkennender Antikörper und Inhibitor-Werkzeugverbindungen werden beschrieben.

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Szewczyk, M. M., Vu, V., Barsyte-Lovejoy, D. Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells. J. Vis. Exp. (174), e62418, doi:10.3791/62418 (2021).

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