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Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells
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Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

3,748 Views

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08:11 min

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August 07, 2021

DOI:

10.3791/62418-v

DOI:
10.3791/62418-v

•

08:11 min
August 07, 2021

•

8 Views
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1Structural Genomics Consortium,University of Toronto, 2Department of Pharmacology and Toxicology,University of Toronto

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These protocols provide the methodology used to assess the enzymatic activity of individual members of the protein arginine methyltransferase (PRMT) family in cells. Detailed guidelines on assessing PRMT activity using endogenous and exogenous biomarkers, methyl-arginine recognizing antibodies, and inhibitor tool compounds are described.

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Szewczyk, M. M., Vu, V., Barsyte-Lovejoy, D. Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells. J. Vis. Exp. (174), e62418, doi:10.3791/62418 (2021).

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