Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Ramona Duman; Christian M. Orr; Vitaliy Mykhaylyk; Kamel El Omari; Robert Pocock; Vinay Grama; Armin Wagner

doi:10.3791/62364

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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

2,376 Views

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10:32 min

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April 23, 2021

DOI:

10.3791/62364-v

DOI:

10.3791/62364-v

•

10:32 min

April 23, 2021

•

5 Views

Ramona Duman¹, Christian M. Orr¹, Vitaliy Mykhaylyk¹, Kamel El Omari¹, Robert Pocock¹, Vinay Grama¹, Armin Wagner¹

¹Diamond Light Source Ltd, Harwell Science & Innovation Campus

Summary

Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.

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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

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