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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

DOI:
10.3791/62364-v

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10:32 min

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April 23, 2021

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, , , , , ,
1Diamond Light Source Ltd, Harwell Science & Innovation Campus

Chapters

  • 00:04Introduction
  • 01:15Crystal Harvesting
  • 01:58Loading of SH from Combipuck to the Transfer Block
  • 03:44Loading of Transfer Blocks into the Vacuum Vessel
  • 08:08Results: Diffraction Images and Structure Solution of Thaumatin
  • 09:41Conclusion

Summary

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Here, we present a protocol for cryogenic sample preparation and transfer of crystals into the vacuum endstation on beamline I23 at Diamond Light Source, for long-wavelength macromolecular X-ray crystallography experiments.

Tags

Keywords: Sample PreparationSample TransferMacromolecular CrystallographyLong-wavelengthBeamline I23Diamond Light SourceCryogenic TemperatureVacuum EnvironmentCombiPuckMagnetic WandLiquid NitrogenDry ShipperSample HolderAdapterPuck Separator Tool

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