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Bepaling van totale lipide- en lipideklassen in mariene monsters
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JoVE Journal Environment
Determination of Total Lipid and Lipid Classes in Marine Samples
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Bepaling van totale lipide- en lipideklassen in mariene monsters

Nederlands

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4,066 Views

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14:59 min

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December 11, 2021

DOI:

10.3791/62315-v

DOI:
10.3791/62315-v

•

14:59 min
December 11, 2021

•

20 Views
,
1Department of Ocean Sciences and CREAIT Network,Memorial University of Newfoundland

Summary

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Dit protocol is voor de bepaling van lipiden in zeewater en biologische specimens. Lipiden in filtraten worden geëxtraheerd met chloroform of mengsels van chloroform en methanol in het geval van vaste stoffen. Lipideklassen worden gemeten door staaf dunnelaagchromatografie met vlamionisatiedetectie en hun som geeft het totale lipidengehalte.

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Cite this Article

Parrish, C. C., Wells, J. S. Determination of Total Lipid and Lipid Classes in Marine Samples. J. Vis. Exp. (178), e62315, doi:10.3791/62315 (2021).

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