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Determination of Total Lipid and Lipid Classes in Marine Samples
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JoVE Journal Environment
Determination of Total Lipid and Lipid Classes in Marine Samples

Determination of Total Lipid and Lipid Classes in Marine Samples

English

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4,066 Views

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14:59 min

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December 11, 2021

DOI:

10.3791/62315-v

DOI:
10.3791/62315-v

•

14:59 min
December 11, 2021

•

20 Views
,
1Department of Ocean Sciences and CREAIT Network,Memorial University of Newfoundland

Summary

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This protocol is for the determination of lipids in seawater and biological specimens. Lipids in filtrates are extracted with chloroform or mixtures of chloroform and methanol in the case of solids. Lipid classes are measured by rod thin-layer chromatography with flame ionization detection and their sum gives the total lipid content.

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Cite this Article

Parrish, C. C., Wells, J. S. Determination of Total Lipid and Lipid Classes in Marine Samples. J. Vis. Exp. (178), e62315, doi:10.3791/62315 (2021).

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