/
/
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
A subscription to JoVE is required to view this content. Sign in or start your free trial.
4,757 Views
•
06:53 min
•
July 10, 2021
DOI:
Summary
Automatically generated
The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.
Read Article
Cite this Article
Succu, S., Serra, E., Gadau, S., Varcasia, A., Berlinguer, F. Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep. J. Vis. Exp. (173), e62272, doi:10.3791/62272 (2021).
Copy