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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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July 10, 2021
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The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.
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Succu, S., Serra, E., Gadau, S., Varcasia, A., Berlinguer, F. Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep. J. Vis. Exp. (173), e62272, doi:10.3791/62272 (2021).
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