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ターナー症候群(45XO)の胎児細胞による、その症候群に伴う神経障害の下流モデリングのための人工多能性幹細胞の生成
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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
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ターナー症候群(45XO)の胎児細胞による、その症候群に伴う神経障害の下流モデリングのための人工多能性幹細胞の生成

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2,898 Views

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09:39 min

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December 04, 2021

DOI:

10.3791/62240-v

DOI:
10.3791/62240-v

•

09:39 min
December 04, 2021

•

8 Views
, , , ,
1Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics,Manipal Hospitals

Summary

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このプロトコルは、核軟部によるエピソームプラスミドの送達を通じた胎児組織線維芽細胞からの統合自由なiPSCの生成と、iPSC特性評価および神経分化に用いられる方法の記述を記述する。

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Cite this Article

Veerasubramanian, N., Karthikeyan, V., Hegde, S., Dhanushkodi, A., Parveen, S. Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome. J. Vis. Exp. (178), e62240, doi:10.3791/62240 (2021).

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