Journal
/
Developmental Biology
/
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

DOI:
10.3791/62240-v

•

09:39 min

•

December 04, 2021

•

, , , ,
1Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics,Manipal Hospitals

Chapters

  • 00:05Introduction
  • 01:12Fibroblast Isolation from Fetal Tissue
  • 02:49Plasmid Isolation and Verification
  • 03:27Nucleofection
  • 06:45iPSC Expansion
  • 07:22iPSC Characterisation
  • 07:34Differentiation to Cells of Three Germ Layers
  • 08:38Results
  • 09:16Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.

Tags

Keywords: Turner Syndrome45XOFetal CellsInduced Pluripotent Stem CellsIPSCsAneuploidReprogrammingChorionic VilliFibroblastsEpisomal PlasmidsCell CultureDifferentiation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/it/62240?article_type=v

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
check_url/it/62240?article_type=v

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
check_url/it/62240?article_type=v

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
check_url/it/62240?article_type=v

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
check_url/it/62240?article_type=v

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
check_url/it/62240?article_type=v

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
check_url/it/62240?article_type=v

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/it/62240?article_type=v

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
check_url/it/62240?article_type=v

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
check_url/it/62240?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article