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クライオ軟X線断層撮影による細胞の3D地図作成記述
JoVE Journal
Biology
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JoVE Journal
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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Please note that all translations are automatically generated.
Click here for the English version.
クライオ軟X線断層撮影による細胞の3D地図作成記述
DOI:
10.3791/62190-v
•
08:47 min
•
March 15, 2021
•
Johannes Groen
,
Andrea Sorrentino
,
Lucía Aballe
,
Robert Oliete
,
Ricardo Valcárcel
,
Chidinma Okolo
,
Ilias Kounatidis
,
Maria Harkiolaki
,
Ana Joaquina Pérez-Berná
,
Eva Pereiro
1
MISTRAL beamline
,
Alba Light Source, Cerdanyola del Valles, Spain
,
2
Beamline B24
,
Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK
Chapters
00:04
Introduction
00:51
Transmission X-Ray Microscope (TXM) Loading
01:49
TXM Brightfield Imaging
02:56
Fluorescence Mode Mosaic Acquisition
03:44
X-Ray Imaging
04:52
Tomography Acquisition
07:07
Results: Representative Cryo Soft X-Ray Tomograms
08:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、クライオ軟X線断層撮影法(SXT)で凍結保存細胞全体の超構造を25nmハーフピッチの分解能で画像化するために必要なサンプル調製およびデータ収集ステップを説明するプロトコルを提示する。
Tags
3D Cartography
Cryo Soft X-ray Tomography
Cellular Imaging
Frozen Hydrated State
High-throughput
Antiviral Drugs
Gene Therapy
Transmission X-ray Microscopy
Cryo Conditions
Sample Loading
Vacuum Procedure
Brightfield Imaging
Sample Rotation
Sample Positioning
Focus Adjustment
Mosaic Acquisition
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