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クライオ軟X線断層撮影による細胞の3D地図作成記述
JoVE Journal
Biology
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JoVE Journal Biology
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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クライオ軟X線断層撮影による細胞の3D地図作成記述

日本語

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3,401 Views

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08:47 min

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March 15, 2021

DOI:

10.3791/62190-v

DOI:
10.3791/62190-v

•

08:47 min
March 15, 2021

•

16 Views
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1MISTRAL beamline,Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24,Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Summary

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ここでは、クライオ軟X線断層撮影法(SXT)で凍結保存細胞全体の超構造を25nmハーフピッチの分解能で画像化するために必要なサンプル調製およびデータ収集ステップを説明するプロトコルを提示する。

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Groen, J., Sorrentino, A., Aballe, L., Oliete, R., Valcárcel, R., Okolo, C., Kounatidis, I., Harkiolaki, M., Pérez-Berná, A. J., Pereiro, E. A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography. J. Vis. Exp. (169), e62190, doi:10.3791/62190 (2021).

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