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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
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Biología
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JoVE Revista Biología
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

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08:47 min

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March 15, 2021

DOI:

10.3791/62190-v

DOI:
10.3791/62190-v

•

08:47 min
March 15, 2021

•

16 Views
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1MISTRAL beamline,Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24,Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Summary

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Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.

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Groen, J., Sorrentino, A., Aballe, L., Oliete, R., Valcárcel, R., Okolo, C., Kounatidis, I., Harkiolaki, M., Pérez-Berná, A. J., Pereiro, E. A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography. J. Vis. Exp. (169), e62190, doi:10.3791/62190 (2021).

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