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ROS Live Cell Imaging During Neuronal Development
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JoVE Journal Neuroscienze
ROS Live Cell Imaging During Neuronal Development

ROS Live Cell Imaging During Neuronal Development

3,969 Views

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09:25 min

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February 09, 2021

DOI:

10.3791/62165-v

DOI:
10.3791/62165-v

•

09:25 min
February 09, 2021

•

4 Views
, ,
1Department of Biological Sciences,Purdue University, 2Purdue Institute for Integrative Neuroscience,Purdue University, 3Bindley Bioscience Center,Purdue University

Summary

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This protocol describes the use of a genetically encoded hydrogen peroxide (H2O2)-biosensor in cultured zebrafish neurons and larvae for assessing the physiological signaling roles of H2O2 during nervous system development. It can be applied to different cell types and modified with experimental treatments to study reactive oxygen species (ROS) in general development.

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Cite this Article

Terzi, A., Alam, S. M. S., Suter, D. M. ROS Live Cell Imaging During Neuronal Development. J. Vis. Exp. (168), e62165, doi:10.3791/62165 (2021).

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